The CellVoyagerTMCV1000, is an fully integrated bench-top spinning disk confocal imaging system equipped with the state of the art Yokogawa CSU spinning disk confocal head, laser illumination, EMCCD camera, laser based autofocus, precision XY and environment control. This cutting edge system enables long term observation of most delicate life processes, including the generation of iPS/ES cells embryogenesis and intracellular communications with ease and without the necessity of dark room operation. The advantage of substantially reduced photo toxicity/bleaching by dual Nipkow disc scanning of the CSU differentiates the CV1000 from any other long term bench top live cell imaging systems.



Principles of the Microlens-enhanced Nipkow Disk Scanning Technology

A Nipkow spinning disk containing about 20,000 pinholes and a second spinning disk containing the same number of microlens to focus excitation laser light into each corresponding pinhole are mechanically fixed with a motor, and very rapidly raster scan the field of view with about 1,000 laser beams when rotated. The pinhole and microlens pattern are arranged in our proprietary design to optimize raster scan. Multi-beam scanning with the CSU-X1 not only increases scanning speed, but also results in significantly lower photobleaching and phototoxicity, because multiple excitation needs only a low level of laser power at the specimen to fully excite fluorescence.

nipkowdisk

Detail of Nipkowdisk method.