Yokogawa CV1000™Applications and Images

High-speed 4D imaging

  • Green: Membrane (EGFP)

Floating 293F cells were transfected with eGFP by using NeoFection, a transfection accelerating agent made by ASTEC.The cells were shake-cultured over night. As a result of time lapse imaging, active movement of cells expressing eGFP inside the floating cell clusters, and structural changes in the cell wall such as ruffing were clearly observed.

ASTEC CO,LTD.

Play full length movie. (1MB)

Imaging Condition
Total Time 20 Hours
Interval 10 minutes
Z-Sections/Stack 101 Sections
(0.3um apart)
Imaging Positions 25 fields
Excitation 488nm
Objective Lens 60x Oil