Yokogawa CV1000™Applications and Images

Embryos Time-Lapse

  • Green: Spindle(EGFP- a-tubulin)
  • Red: Nucleus(H2B-mRFP1)

Following the injection of mouse embryos with mRNA, nearly 60,000 multicolor and multilayer confocal images of the embryos were acquired over a 70-hour period as they developed to the blastocyst stage.

Kazuo Yamagata, PhD., Wakayama Lab.(Laboratory for genomic Reprogramming), Center for Development Biology, RIKEN

Play full length movie. (10MB)

Imaging Condition
Total Time 60 Hours
(2.5 days)
Z-Sections/Stack 51 Sections
(2um apart)
Imaging Positions 6 fields
(72 embryos)
Excitation 488nm, 561nm
Objective Lens 20x Oil