bioimagingsolutions

Molecular cloning and functional characterization of TaIRI9 gene in wheat

Molecular cloning and practical characterization of TaIRI9 gene in wheat (Triticum aestivum L.)

The vernalization of wheat is among the vital components that decide the planting area, introduction and cultivation methods of wheat. Nonetheless, the recognized vernalization genes (molecular marker) can not exactly distinguish the vernalization requirement of winter wheat cultivars.

Subsequently, it is very important discover new vernalization genes and elucidate the mechanism of vernalization regulation. To discover the gene community within the vernalization pathway, we screened TaIRI9 (ice recrystallization inhibitor protein) gene related to the expression profile of vernalization remedy of winter wheat Jing 841. Overexpression of TaIRI9 in wild sort wheat resulted in diminished plant peak, elevated tiller quantity and delayed heading days.

After 4°C vernalization remedy for 30, 35, 45 or 50 days, TaIRI9 overexpression traces confirmed elevated vernalization requirement and delayed heading time than wild sort, indicating that TaIRI9 might have an effect on vernalization strategy of wheat. As well as, the expression of the TaIRI9 genes have been analyzed in winter Jing 841, sturdy winter wheat cultivar Xindong 18 and ten recombinant inbred traces (RILs, Hussar x Yanzhan1).

The information confirmed that the expression of TaIRI9 was positively related to the requirement of vernalization. These outcomes indicated that TaIRI9 regulates heading and flowering time in wheat by selling VRN2 and inhibiting flowering promoter VRN1 and VRN3 and could also be concerned in wheat vernalization regulation pathway. Bulked segregant CGT-Seq-facilitated map-based cloning of a powdery mildew resistance gene originating from wild emmer wheat (Triticum dicoccoides)

Powdery mildew, brought on by Blumeria graminis f. sp. tritici (Bgt), is a broadly occurring foliar illnesses of wheat worldwide. Wild emmer wheat (WEW, Triticum dicoccoides) (AABB, 2n=4x=28), the progenitor of the cultivated tetraploid and hexaploid wheat, is extremely immune to powdery mildew and plenty of resistance alleles have been recognized on this wild species.

Molecular cloning and characterization of a thermostable and halotolerant endo-β-1,4-glucanase from Microbulbifer sp. ALW1

The bacterium Microbulbifer sp. ALW1 was beforehand characterised with the aptitude to interrupt down the cell wall of brown algae into superb items. The organic features of pressure ALW1 have been but to be elucidated. On this examine, a gene, specifically MaCel5A, was remoted from the ALW1 pressure genome, encoding an endo-β-1,4-glucanase. MaCel5A was phylogenetically categorized below the glycoside hydrolase household GH5, with the very best identification to a putative cellulase of Microbulbifer thermotolerans. The recombinant MaCel5A protein purified from heterologous expression in E. coli exhibited most exercise at 50 °C and pH 6.0, respectively, and functioned selectively towards carboxymethyl cellulose and barley β-glucan.

Recombinant MaCel5A demonstrated appreciable tolerance to the publicity to excessive temperature as much as 80 °C for 30 min retaining 49% residual exercise. As well as, MaCel5A confirmed average stability towards pH 5.0-11.Zero and powerful stability within the presence of nonionic surfactant. MaCel5A exhibited sturdy halo-stability and halotolerance. The exercise of the enzyme elevated about tenfold at 0.5 M NaCl, and about fivefold even at 4.Zero M NaCl in comparison with the enzyme exercise with out the addition of salt. The 2 conserved glutamic acid residues in MaCel5A featured the standard catalytic acid/base and nucleophile equipment of glycoside hydrolases. These traits spotlight the commercial software potential of MaCel5A.

bioimagingsolutions
bioimagingsolutions

Fusion (FUS) Antibody

20-abx100040
  • EUR 526.80
  • EUR 159.60
  • EUR 1479.60
  • EUR 710.40
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion (FUS) Antibody

20-abx176518
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Fusion (FUS) Antibody

20-abx172487
  • EUR 1028.40
  • EUR 526.80
  • 1 mg
  • 200 ug

Fusion (FUS) Antibody

20-abx212423
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Fusion (FUS) Antibody

20-abx212424
  • EUR 493.20
  • EUR 360.00
  • 100 ul
  • 50 ul

Fusion (FUS) Antibody

20-abx129140
  • EUR 510.00
  • EUR 159.60
  • EUR 1446.00
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Morpheus Fusion FX

M-MD1-130-FX 96 x 100 ul ul
EUR 67
Description: Morpheus Fusion FX

Recombinant Fusion (FUS)

4-RPC260Hu01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Fusion expressed in: E.coli

Recombinant Fusion (FUS)

4-RPC260Mu01
  • EUR 603.84
  • EUR 285.60
  • EUR 1934.40
  • EUR 724.80
  • EUR 1329.60
  • EUR 480.00
  • EUR 4656.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Fusion expressed in: E.coli

TIM-3, Fc fusion

71151 100 µg
EUR 320
Description: Human secreted TIM-3, Fc fusion protein, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, T-cell and immunoglobulin and mucin domain-containing protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, and HAVCR-2. GenBank Accession No. NM_032782.4, a.a. 22-200 expressed in a HEK293 cell expression system. MW = 46.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Human LTBR, Fc fusion

71112 100 µg
EUR 310
Description: Human secreted lymphotoxin beta receptor_x000D_(LTBR)-Fc fusion protein, also known as_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_002342, a.a 28-219, expressed in_x000D_a HEK293 cell expression system. MW = 48_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Mouse LTBR, Fc fusion

71122 100 µg
EUR 340
Description: Mouse secreted lymphotoxin beta receptor_x000D_(mLTBR)-Fc fusion protein, also known as,_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_010736, a.a 28-221, expressed in_x000D_a HEK293 cell expression system. MW =_x000D_48.8 kDa (monomer). Endotoxin level_x000D_<0.001 EU/ug.This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

BTLA(CD272), Fc fusion

71141 100 µg
EUR 320
Description: Human B- and T-lymphocyte attenuator (BTLA)-Fc fusion protein, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.

LAG3 (CD223), Fc fusion

71146 100 µg
EUR 320
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system. MW = 73.1 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

GITR (CD357), Fc fusion

71172 100 µg
EUR 300
Description: Human secreted GITR, Fc fusion protein, also known as Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, GenBank Accession No. NM_004195, a.a. 26-161 expressed in a HEK293 cell expression system. MW = 41.2 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

OX40 (CD134), Fc fusion

71175 100 µg
EUR 320
Description: Human secreted OX40, Fc fusion protein,_x000D_also known as Tumor Necrosis Factor_x000D_Receptor Superfamily Member 4,_x000D_TNFRSF4, and CD134, GenBank_x000D_Accession No. NM_003327, a.a. 29-216_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 46.8 kDa (monomer). This_x000D_protein runs at a higher MW by SDS-PAGE_x000D_due to glycosylation.

ICOS (CD278), Fc fusion

71179 100 µg
EUR 320
Description: Human ICOS, Fc fusion protein, also known as inducible T-cell costimulator and CD antigen 278, activation-inducible lymphocyte immunomediatory molecule, AILIM, CD278, and CVID1. GenBank Accession No. NM_012092, a.a. 21-140 expressed in a HEK293 cell expression system. MW = 40.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Fusion 1 (Fus1) Antibody

20-abx121804
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

ACE2, Fc Fusion (Monkey)

100701-1 50 µg
EUR 490
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.

ACE2, Fc Fusion (Monkey)

100701-2 1 mg
EUR 3200
Description: Rhesus monkey angiotensin I converting enzyme 2 (ACE2), also known as ACEH, Genbank Accession No.: ACI04553.1, a.a. 18-739, fused at the C-terminus of the Fc portion of human IgG1, expressed in a HEK293 expression system, MW= 119 kDa. This protein runs at a higher MW due to glycosylation.

CTLA4 (CD152), Fc fusion

71149-1 100 µg
EUR 150
Description: Human secreted CTLA4 , Fc fusion protein, also known as Cytotoxic T-lymphocyte-associated protein 4 and CD152. GenBank Accession No. NM_005214, a.a. 36-162 expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Human CD226, Fc fusion

71252 100 µg
EUR 320
Description: Human CD226 antigen also_x000D_known as DNAX accessory molecule 1_x000D_(DNAM-1), GenBank Accession No._x000D_NM_006566, a.a. 19-247 fused to Fc region_x000D_of human IgG1, expressed in a HEK293 cell_x000D_expression system. MW = 52.8 kDa_x000D_(monomer). This protein runs at a higher_x000D_MW by SDS-PAGE due to glycosylation.

Fusion (FUS) Antibody (HRP)

20-abx107842
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Frizzled-1, Fc fusion

71123 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD1)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD1, GenBank Accession No.BC_051271,_x000D_a.a. 73-253, expressed in a HEK293 cell_x000D_expression system. MW = 46.4 kDa_x000D_(monomer). This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Frizzled-4, Fc fusion

71126 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD4)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD4, GenBank Accession No._x000D_BC_114527, a.a. 37-180, expressed in a_x000D_HEK293 cell expression system. MW = 42.9_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Frizzled-7, Fc Fusion

71139 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD7)-Fc fusion_x000D_protein and WNT receptor, also known as_x000D_FZD7, GenBank Accession No._x000D_BC_015915, a.a. 33-185, expressed in a_x000D_HEK293 cell expression system. MW = 43.3_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Fusion (FUS) Antibody (FITC)

20-abx271237
  • EUR 594.00
  • EUR 309.60
  • EUR 1746.00
  • EUR 811.20
  • EUR 477.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion (FUS) Antibody (FITC)

20-abx106427
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Human Fusion (FUS) Protein

20-abx168102
  • EUR 777.60
  • EUR 326.40
  • EUR 2397.60
  • EUR 927.60
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Mouse Fusion (FUS) Protein

20-abx066708
  • EUR 844.80
  • EUR 343.20
  • EUR 2598.00
  • EUR 994.80
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Frizzled-10, Fc fusion

71124 50 µg
EUR 325
Description: Secreted frizzled receptor (FZD10)-Fc_x000D_fusion protein and WNT receptor, also_x000D_known as FZD10, GenBank Accession No._x000D_BC_074997, a.a. 21-161, expressed in a_x000D_HEK293 cell expression system. MW = 42.7_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Morpheus Fusion HT-96

M-MD1-130 96 x 1 ml ml
EUR 317
Description: Morpheus Fusion HT-96

UIM-UBA Fusion Protein

6571-250 each
EUR 326.4

B7-1 (CD80), Fc fusion

71125 100 µg
EUR 320
Description: Human secreted B7-1-Fc fusion protein, also known as CD80. GenBank Accession No. NM_005191, a.a. 35-242 expressed in a HEK293 cell expression system. MW = 50.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.

B7-2 (CD86), Fc fusion

71150 100 µg
EUR 320
Description: Human secreted B7-2, Fc fusion protein, also known as T-lymphocyte activation antigen CD86, B7.2, FUN-1, B70, BU63, and CD86. GenBank Accession No. NM_006889, a.a. 20-239 expressed in a HEK293 cell expression system. MW = 51.9 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Fusion (FUS) Antibody (Biotin)

20-abx271503
  • EUR 560.40
  • EUR 292.80
  • EUR 1612.80
  • EUR 760.80
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion (FUS) Antibody (Biotin)

20-abx105013
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

CD152 Mulg Fusion protein

30R-CD152 25 ug
EUR 805.2
Description: Purified recombinant Human CD152 Mulg Fusion protein

PD-1/PDCD1(Fc fusion)

E21-J97 10ug
EUR 411.6

Fusion 1 Blocking Peptide

20-abx161514
  • EUR 727.20
  • EUR 1713.60
  • 1 mg
  • 5 mg

B7-H5 (VISTA), Fc fusion

71148 100 µg
EUR 320
Description: Human secreted B7-H5, Fc fusion protein, also known as Gi24, Dies1, VISTA, and SISP1, GenBank Accession No. NM_022153, a.a. 33-194 expressed in a HEK293 cell expression system. MW = 46.7 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Human Fusion (FUS) CLIA Kit

20-abx493573
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Fusion (FUS) CLIA Kit

20-abx493574
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

VCAP18/GP125 fusion protein

30-1296 1 mg
EUR 4177.2
Description: Purified recombinant VCAP18/GP125 fusion protein

RSV Fusion Protein Antibody

abx022172-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022173-1mg 1 mg
EUR 1387.2

RSV Fusion Protein Antibody

abx022174-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022175-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022176-1mg 1 mg
EUR 1296

RSV Fusion Protein Antibody

abx022177-1mg 1 mg
EUR 693.6

RSV Fusion Protein Antibody

abx022179-100ug 100 ug
EUR 978

MPV Fusion Protein Antibody

abx023874-1mg 1 mg
EUR 1178.4

RSV Fusion glycoprotein [His]

DAG-H10334 10 µg
EUR 868.8

MICA, Fc fusion, Avi-Tag

100295 100 µg
EUR 320
Description: Human MICA, also known as, MHC Class I Polypeptide-Related Sequence A, PERB11.1, and Stress Inducible Class I Homolog, GenBank Accession No. NM_001177519, a.a. 23-308, fused at the C-terminus to the Fc portion of human IgG1 followed by a C-terminal Avi-Tag™ and expressed in a HEK293 cell expression system. MW=62 kDa.

BTLA, Fc Fusion, Avi-Tag

100033 100 µg
EUR 320
Description: Human B- and T-lymphocyte attenuator (BTLA), also known as CD272, with C-terminal Avi-TagTM fused to the Fc-region of Human IgG1. GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 42 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.

CD33, Fc Fusion, Avi-tag

100155 100 µg
EUR 320
Description: Human secreted CD33, also known as Sialic Acid Binding Ig Like Lectin 3, Gp67 and Myeloid Cell Surface Antigen CD33, GenBank Accession No. NM_001772, a.a. 1-259, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™ , expressed in a HEK293 cell expression system. MW = 57 kDa.

CD24, Fc Fusion, Avi-tag

100527-1 100 µg
EUR 320
Description: Human secreted CD24, also known as Signal Transducer CD24, Small Cell Lung Carcinoma Cluster 4 Antigen and CD24A, GenBank Accession No. NM_013230, a.a. 27-59(end), fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system. MW = 32 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

CD24, Fc Fusion, Avi-tag

100527-2 1 mg
EUR 2595
Description: Human secreted CD24, also known as Signal Transducer CD24, Small Cell Lung Carcinoma Cluster 4 Antigen and CD24A, GenBank Accession No. NM_013230, a.a. 27-59(end), fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system. MW = 32 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

EPOR, Fc fusion, Avi-Tag

100611 20 µg
EUR 320
Description: Human Erythropoietin Receptor, also known as EPOR, GenBank Accession No. NM_000121, a.a 25-250, fused at the C-terminus of the Fc portion of human IgG1, with C-terminal Avi-tag™, expressed in a HEK293 cell expression system, MW=54 kDa.

Human Fusion (FUS) ELISA Kit

20-abx151603
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Fusion (FUS) ELISA Kit

20-abx154044
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Fusion (FUS) ELISA Kit

DLR-FUS-Hu-48T 48T
EUR 620.4
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.

Human Fusion (FUS) ELISA Kit

DLR-FUS-Hu-96T 96T
EUR 807.6
Description: A sandwich quantitative ELISA assay kit for detection of Human Fusion (FUS) in samples from tissue homogenates or other biological fluids.

Mouse Fusion (FUS) ELISA Kit

DLR-FUS-Mu-48T 48T
EUR 632.4
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.

Mouse Fusion (FUS) ELISA Kit

DLR-FUS-Mu-96T 96T
EUR 825.6
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Fusion (FUS) in samples from tissue homogenates or other biological fluids.

Human Fusion (FUS) ELISA Kit

SEC260Hu-10x96wellstestplate 10x96-wells test plate
EUR 5677.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.

Human Fusion (FUS) ELISA Kit

SEC260Hu-1x48wellstestplate 1x48-wells test plate
EUR 572.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.

Human Fusion (FUS) ELISA Kit

SEC260Hu-1x96wellstestplate 1x96-wells test plate
EUR 766.8
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.

Human Fusion (FUS) ELISA Kit

SEC260Hu-5x96wellstestplate 5x96-wells test plate
EUR 3090.6
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Fusion (FUS) in tissue homogenates, cell lysates and other biological fluids.

Human Fusion (FUS) ELISA Kit

4-SEC260Hu
  • EUR 5738.40
  • EUR 3031.20
  • EUR 768.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Fusion (FUS) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Fusion (FUS) ELISA Kit

SEC260Mu-10x96wellstestplate 10x96-wells test plate
EUR 5834.88
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.

Mouse Fusion (FUS) ELISA Kit

SEC260Mu-1x48wellstestplate 1x48-wells test plate
EUR 585.7
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.

Mouse Fusion (FUS) ELISA Kit

SEC260Mu-1x96wellstestplate 1x96-wells test plate
EUR 785.28
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.

Mouse Fusion (FUS) ELISA Kit

SEC260Mu-5x96wellstestplate 5x96-wells test plate
EUR 3173.76
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Fusion (FUS) in Tissue homogenates and other biological fluids.

Mouse Fusion (FUS) ELISA Kit

4-SEC260Mu
  • EUR 5895.60
  • EUR 3114.00
  • EUR 786.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Fusion (FUS) in samples from Tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.

Human Fusion ELISA Kit (FUS)

RK01425 96 Tests
EUR 625.2

Human Fusion (FUS) ELISA Kit

RDR-FUS-Hu-48Tests 48 Tests
EUR 652.8

Human Fusion (FUS) ELISA Kit

RDR-FUS-Hu-96Tests 96 Tests
EUR 907.2

Mouse Fusion (FUS) ELISA Kit

RDR-FUS-Mu-48Tests 48 Tests
EUR 668.4

Mouse Fusion (FUS) ELISA Kit

RDR-FUS-Mu-96Tests 96 Tests
EUR 928.8

Human Fusion(FUS)ELISA Kit

QY-E01737 96T
EUR 433.2

Human Fusion (FUS) ELISA Kit

RD-FUS-Hu-48Tests 48 Tests
EUR 625.2

Human Fusion (FUS) ELISA Kit

RD-FUS-Hu-96Tests 96 Tests
EUR 867.6

Mouse Fusion (FUS) ELISA Kit

RD-FUS-Mu-48Tests 48 Tests
EUR 639.6

Mouse Fusion (FUS) ELISA Kit

RD-FUS-Mu-96Tests 96 Tests
EUR 888

Human Fusion (FUS) ELISA Kit

RD-FUS-Hu-48T 48T
EUR 452.7
Description: tissue homogenates and other biological fluids.

Human Fusion (FUS) ELISA Kit

RD-FUS-Hu-96T 96T
EUR 646.7
Description: tissue homogenates and other biological fluids.

Mouse Fusion (FUS) ELISA Kit

RD-FUS-Mu-48T 48T
EUR 464.3
Description: tissue homogenates and other biological fluids.

Mouse Fusion (FUS) ELISA Kit

RD-FUS-Mu-96T 96T
EUR 663.3
Description: tissue homogenates and other biological fluids.

Human Fusion (FUS) ELISA Kit

RDR-FUS-Hu-48T 48T
EUR 475.34
Description: tissue homogenates and other biological fluids.

Cloning and bodily localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae)

Spinach (Spinacia oleracea Linnaeus, 1753) is a perfect materials for finding out molecular mechanisms of early-stage intercourse chromosome evolution in dioecious vegetation. Degenerate oligonucleotide-primed polymerase chain response (DOP-PCR) approach facilitates the retrotransposon-relevant research by enriching particular repetitive DNA sequences from a micro-dissected single chromosome. We carried out genomic subtractive hybridization to display screen sex-biased DNA sequences by utilizing the DOP-PCR amplification merchandise of micro-dissected spinach Y chromosome.

The screening yielded 55 male-biased DNA sequences with 30 576 bp in size, of which, 32 DNA sequences (12 049 bp) contained repeat DNA sequences, together with LTR/CopiaLTR/Gypsy, easy repeats, and DNA/CMC-EnSpm. Amongst these repetitive DNA sequences, 4 DNA sequences that contained a fraction of Ty3-gypsy retrotransposons (SP73, SP75, SP76, and SP77) have been chosen as fluorescence probes to hybridization on female and male spinach karyotypes.

Fluorescence in situ hybridization (FISH) alerts of SP73 and SP75 have been captured totally on the centromeres and their surrounding space for every homolog. Hybridization alerts primarily appeared close to the putative centromeres for every homologous chromosome pair by utilizing SP76 and SP77 probes for FISH, and sporadic alerts existed on the lengthy arms. Outcomes might be served as a foundation to check the operate of repetitive DNA sequences in intercourse chromosome evolution in spinach.

Cloning and characterization of a novel DNase gene from Trichogramma pretiosum

DNase is a robust instrument for a sequence of molecular biology functions. Growing a technique for large-scale manufacturing of DNase with excessive purity and exercise is crucial for scientific analysis. On this examine, a beforehand uncharacterized gene with nuclease exercise was present in Trichogramma pretiosum genome. Pichia pastoris GS115 was most popular because the host to beat the problems associated to prokaryotic expression. Below the optimum circumstances, the exercise of T. pretiosum DNase (Tp-DNase) reached 1940 U/mL of tradition supernatant in fed-batch fermentation. Utilizing ion-exchange chromatography and adsorption chromatography, Tp-DNase was produced with a purity of > 99% and molecular weight of 45 kDa.

In vitro DNA degradation experiments confirmed that Tp-DNase may successfully degrade dsDNA, and its exercise was barely larger than that of bovine pancreas DNase I below the identical circumstances. Furthermore, Tp-DNase can be utilized to eradicate nucleic acid contamination and enhance the accuracy of nucleic acid detection.

Cloning, expression, and characterization of Baeyer-Villiger monooxygenases from eukaryotic Exophiala jeanselmei pressure KUFI-6N

The fungus Exophiala jeanselmei pressure KUFI-6N produces a singular cycloalkanone monooxygenase (ExCAMO) that shows an unusual substrate spectrum of Baeyer-Villiger oxidation of 4-10-membered ring ketones. On this examine, we aimed to determine and sequence the gene encoding ExCAMO from KUFI-6N and overexpress the gene in Escherichia coli. We discovered that the first construction of ExCAMO is most intently associated to the cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011, with 54.2% amino acid identification. ExCAMO was functionally expressed in Escherichia coli and its substrate spectrum and kinetic parameters investigated.

Substrate profiling indicated that ExCAMO is uncommon amongst recognized Baeyer-Villiger monooxygenases owing to its capability to just accept a wide range of substrates, together with C4-C12 membered ring ketones. ExCAMO has excessive affinity and catalytic effectivity towards cycloalkanones, the very best being towards cyclohexanone. 5 different genes encoding Baeyer-Villiger monooxygenases have been additionally cloned and expressed in Escherichia coli.